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Southern blot analysis of B. aphidicola (P. spyrothecae) plasmid DNA. Hybridizations were performed with a probe complementary to trpG. Restriction enzymes: A, AccI; P, PvuII; B, BamHI; H, HindIII; V, EcoRV; C, ClaI; X, XbaI; E, EcoRI; T, PstI; K, ScaI; O, XhoI. oc, open circular; L, linear; ccc, covalently closed circular.
