Table 1.

EMR mass spectrometer tune settings used in this method

Setting	Value	Notes
Spray voltage (kV)	0.7–0.8	First increase spray voltage past the recommended range, then gradually decrease it until a stable signal is observed.
Capillary temperature (°C)	250
S-Lens RF level (V)	200
Injection flatapole DC (V)	12
Bent flatapole DC (V)	10
Inter flatapole lens (V)	8
HCD direct eV (V)	1	Higher HCD voltages can be used for fragmentation and for de-adducting.
Inject time (ms)	200
Microscans	2
Scan range (m/z)	1,000–10,000
Resolution (at 200 m/z)	8,750	Adjust to higher resolution to resolve isotopic peaks.
Trapping gas pressure	4–6	Use larger values to improve higher m/z transmission.