Component	Initial concentration	Volume (μL)	Final concentration
Reaction buffer	-	5	-
Folded RPR	6.25 nM	2	0.625 nM
POP5·RPP30	312.5 nM	2	31.25 nM
RPP21	312.5 nM	2	31.25 nM
RPP29	312.5 nM	2	31.25 nM
L7Ae	312.5 nM	2	31.25 nM
Pre-tRNA mix	2 μM; 1,600 dpm/μL	5	500 nM; 400 dpm/μL*

^*Quenching 2.5–5 μL will allow loading of 1,000–2,000 disintegrations per minute (dpm) (see Note 23)