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. 2022 Apr 29;16:882366. doi: 10.3389/fncir.2022.882366

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Copyright © 2022 Hui, Zheng, Zhang, Li, Yu, Li, Zhang, Gong and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The schematic depiction of the Cre-LoxP system. (A) Excision: Cre recombinase cuts the coding sequences between loxP sites in the same orientation. (B) Inversion: Cre recombinase leads to the inversion of coding sequences between loxP sites in the opposite orientation. (C) Induced expression: Cre recombinase excises the loxP-flanked “STOP” sequence to induce the expression of the transgene. (D) In the Flip-Excision (FLEX) switch system, loxP and lox2272 are heterotypic and antiparallel; therefore, after the inversion and subsequent excision, the expression of transgene can be achieved through Cre-mediated inversion of the reverse-constructed coding sequence (Adapted from Atasoy et al., 2008).