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. 2022 May 8;23(9):5241. doi: 10.3390/ijms23095241

Figure 1.

Figure 1

Characteristics of PalERF2 of Populus alba var. Pyramidalis. (A) Multiple sequence alignment of ERFs and the accession numbers are derived from different species (NM_001325036.1 from Nicotiana tabacum, XP_015636763.1 from rice, AT5G47220.1 from Arabidopsis thaliana, KAB5574006.1 from Salix brachista, and XP_003538752.2 from Glycine max). (B) The qRT-PCR analysis of PalERF2 expression in root (R), stem (S), mature leaves (ML), young leaves (YL), and petiole (P) in the mediums of MS. (C,D) The temporal expression pattern of PalERF2 under low Pi treatment (10 μM Pi) and 150 mM mannitol treatment in shoot and root, respectively. Error bars indicate SD values from three biological replicates. (E) Subcellular localization of PalERF2 in the mesophyll protoplasts of P. alba var. pyramidalis. The empty vector pBI221-expressing GFP is used as control (upper row) and the PalERF2-GFP fusion proteins are localized in the nucleus only (lower row). DAPI staining indicates the nucleus.