Table A1.

Biomarker details.

Biomarkers	Symbol	Unit	Method of Measurements/Name of the Machine and Related Information	Analytical Detection Limit	CHMS Reference Laboratory Precision Target
Serum 25-hydroxyvitamin D [2,54]	S-25(OH)D	nmol/L	Chemiluminescent immunoassay technology (DiaSorin, Ltd., Stillwater, MN, USA)	10–375	<20 nmol/L = 15% 20–100 nmol/L = 10% >100 nmol/L = 12%
Glycated hemoglobin	HbA1c	%	Immunoturbidimetric end-point spectrophotometry (anti-HbA1c antibody, polyhapten) on the Ortho Clinical Diagnostics Vitros 5, 1FS analyzer. %A1c is a derived test calculated from the quantitative measurements of hemoglobin and hemoglobin A1c	Hb: 6.0–22.0 g/dL(component) HbA1c: 0.2–2.5 g/dL (component) %A1c: 4–14% NGSP	5%
Total cholesterol/HDL cholesterol ratio	TC/HDL ratio		Derived calculation	N/A	N/A
High-sensitivity C-reactive protein	hs-CRP	mg/L	Immunoturbidimetric two-point rate reflectance spectrophotometry (monoclonal antibodies) on the Ortho Clinical Diagnostics Vitros 5, 1FS analyzer	0.10–15.0	5%
Total immunoglobulin E	IgE	IU/mL	Two-site sandwich immunoassay using direct chemiluminescent technology on the Siemens ADVIA Centaur XP analyzer	1.5–3300	10%
Ferritin	Ferritin	µg/L	Two-site sandwich immunoassay using direct chemiluminescent technology on the Siemens ADVIA Centaur XP analyzer	0.5–1650	10%
Hemoglobin	Hb	g/L	Hemoglobin is a non-cyanide-based method read photometrically at 555 nm on the POCH-i100 Sysmex hematology analyzer	0–25.0	1.5%