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. 2022 May 6;23(9):5203. doi: 10.3390/ijms23095203

Figure 2.

Figure 2

Effects of IDO1 genetic ablation on NASH and atherosclerosis development. Apoe−/− or Apoe−/−Ido1−/− mice were fed a high-fat cholesterol diet (HFCD) for 7 weeks; pooled data from two independent experiments are shown. (A) Quantification of en face Sudan IV-stained aortic arches (n = 14–15). (B) Representative pictures of the aortic arches. (C) Representative pictures of the atherosclerotic burden stained by ORO in aortic root sections of Apoe−/− or Apoe−/− Ido1−/− mice (n = 2/group). (D) Representative picture of Mac-2+ macrophage infiltration in the aortic roots of Apoe−/− or Apoe−/− Ido1−/− mice (n = 2/group). Panel (E) shows total cholesterol and (F) triglyceride levels in plasma (n = 15–16). (G,H) Total levels of cholesterol and triglycerides in Apoe−/− and Apoe−/−Ido1−/− mice livers (n = 15–16). (I) Representative pictures of H&E-stained liver sections. (J) Liver/body ratio (n = 15–16), (K,L) plasma ALT and AST levels (n = 15–16). (M) Relative hepatic collagen (Col1a1) mRNA expression (n = 15–16) and (N) hepatic hydroxyproline levels (n = 15–16). (O) Representative pictures of picrosirius red-stained liver sections. ** p < 0.01; differences were detected using the Mann–Whitney U test; dotted lines refer to baseline levels of Apoe−/− mice fed a chow diet for 7 weeks (Table 1); orange and blue colours are used to identify female and male mice, respectively. Bar = 50 µm.