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. 2022 Apr 26;14(9):2151. doi: 10.3390/cancers14092151

Figure 4.

Figure 4

Effects of SNAT1 downregulation on migratory and invasive potential of melanoma cells. (A,B) Analysis of cell migration using scratch wound healing assay of Mel Im and Mel Juso cell lines after siSNAT1 downregulation when compared to siCTR cells. (A) Representative images of cell monolayer scratch of Mel Im cells 24 h and of Mel Juso cells 48 h after scratching. (B) Scratch width of Mel Im cells 24 h and of Mel Juso cells 48 h after scratching (siCTR = 1). Values represent the mean ± SEM of 4 independent experiments. (C,D) Analysis of cellular migration using Boyden chamber assay of Mel Im and Mel Juso cell lines after siRNA-mediated SNAT1 downregulation, compared to siCTR-transfected melanoma cells. (C) Representative images of Boyden chamber gelatine-coated filter membrane. (D) Quantification of migrated cells (siCTR = 1). Values represent the mean ± SEM of 4 independent experiments. (E,F) Analysis of cellular invasion using Boyden chamber assay of Mel Im and Mel Juso cell lines after siRNA-mediated SNAT1 downregulation, compared to siCTR-transfected melanoma cells. (E) Representative images of Boyden chamber Matrigel-coated filter membrane. (F) Quantification of invasive cells (siCTR = 1). Values represent the mean ± SEM of 4 independent experiments. p-value < 0.05 was considered statistically significant (*).