Figure 7.
Anti-inflammatory activity of FCNG in primary human articular chondrocytes. (A) Chondrocytes were pre-treated with (11.7 ± 4.5) × 109 particles/mL of FCNP and FCNG for 24 h, or dexametasone (DXM, 2 µM), followed by stimulation with IL-1β (10 ng/mL) for an additional 24 h, and analyzed for levels of IL-6 present in the cell culture media by ELISA. Non-stimulated cells were used as controls of IL-1β stimulation. (B) Viability of articular chondrocytes exposed to (11.7 ± 4.5) × 109 particles/mL of FCNP or FCNG for 48 h. Data are representative of three independent experiments and are presented as mean ± SD. Two-way ANOVA and multiple comparisons were performed with the Dunnett’s test, and are presented relative to the IL-1β-stimulated cells. Statistical significance was defined as p ≤ 0.05 (*), p ≤ 0.01 (**), and p ≤ 0.0001 (****).