Table 2.
Results of 5mC and 5hmC measurements in three subpopulations of spermatozoa fractionated according to their chromatin protamination status.
| Subpopulation | Control Group: Mean C ± SD |
Patients’ Group: Mean P ± SD |
|
|---|---|---|---|
| 5mC [IF i.u.] | protaminated [pink] | 120.28 ± 19.65 | 87.29 * ± 36.01 |
| semiprotaminated [purple] | 58.92 ± 15.41 | 59.54 ± 26.34 | |
| deprotaminated [navy blue] | 37.90 ± 8.84 | 42.73 ± 20.11 | |
| mean of semi- and deprotaminated | 48.41 ± 10.23 | 51.13 ± 22.72 | |
| 5hmC [IF i.u.] | protaminated [pink] | 134.26 ± 15.12 | 121.20 ± 23.42 |
| semiprotaminated [purple] | 110.85 ± 11.48 | 100.76 ± 24.95 | |
| deprotaminated [navy blue] | 98.71 ± 9.97 | 81.53 ± 22.39 | |
| mean of semi- and deprotaminated | 104.78 ± 10.51 | 91.15 ± 23.03 | |
| 5mC/5hmC ratio | protaminated [pink] | 0.90 ± 0.12 | 0.70 ** ± 0.23 |
| semiprotaminated [purple] | 0.53 ± 0.12 | 0.58 ± 0.20 | |
| deprotaminated [navy blue] | 0.38 ± 0.07 | 0.52 * ± 0.19 | |
|
mean of semi- and
deprotaminated |
0.46 ± 0.07 | 0.55 ± 0.19 |
Mean P-values statistically significant (p < 0.05) from mean C values are marked with * for p < 0.05, and ** for p < 0.01; at least 50 spermatozoa were analysed per population in each case.