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. 2022 May 2;14(9):2272. doi: 10.3390/cancers14092272

Figure 5.

Figure 5

Potential upstream targets for the protective effects of nicotine and cotinine in SCLC against cisplatin treatment. (A) The effects of nAChR inhibition (MEC) on nicotine or cotinine-induced cisplatin resistance. (B) The levels of CHRNA4, CHRNA3, and CHRNB2 mRNAs in SCLC cell lines, compared to NSCLC cell lines. (C) CHRNA4, CHRNA3, and CHRNB2 mRNA levels are upregulated upon cisplatin treatment in H841. (D) CHRNA3 mRNA level correlates positively with carboplatin resistance among SCLC cell lines. (E) H841, (F) DMS53, (G) DMS114, and (H) H446 exhibit significant increases in CHRNA4, CHRNA3, and CHRNB2 mRNA levels upon cisplatin or cotinine + cisplatin treatment, but with minimal and insignificant changes in (I) H1048, (J) H146, (K) H372, and (L) H526 cells. Statistically significant differences determined using t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001 and ****, p < 0.0001.