Figure 7.

The effects of nicotine on chemoresistance of H841 SCLC tumors to cisplatin in a mouse xenograft model (n = 5). (A) Body weight changes during the experimental period (measured twice per week). (B) Xenograft tumor growth curves. (C) The final tumor weights. (D) Final tumor images with different treatments. (E) Western blotting used to determine the protein levels of p-ERK, ERK, p-AKT, AKT, cleaved-PARP, γ-H2AX, and PCNA with β-actin as the loading control in the combined tumor samples from each group. (F) The levels of CHRNA4, CHRNA3, and CHRNB2 mRNA in tumor samples. t test used for statistic quantifications: * p < 0.05, ** p < 0.01, **** p < 0.0001, respectively. The whole blots could be found in the Figure S6.