Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 19;14(9):2055. doi: 10.3390/cancers14092055

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Contribution of H19 to EMT and invasion in PDAC sphere cells. PANC-1 cells stably transfected with mock or H19 expression vector were cultured for 4 days in 3D (sphere) conditions with or without 1 μM SC144 and then further incubated for 3 days with or without 10 ng/mL of TGF-β1. The cells were harvested and then used in the following experiments. (A) Real-time qPCR analysis of H19. Results are normalized to values obtained for mock-transfected sphere cells (value = 1). Results are presented as means ± SD from three independent experiments. (B) Real-time qPCR analysis of EMT markers. Results are normalized to values obtained for mock-transfected sphere cells (value = 1). Results are presented as means ± SD from three independent experiments. (C) Real-time qPCR analysis of MT1-MMP and MMP2. Results are normalized to values obtained for mock-transfected sphere cells (value = 1). Results are presented as means ± SD from three independent experiments. (D) 3D-invasion assay. Scale bar: 5 μm. The histograms indicate the invaded area. Results are presented as means ± SD from four sphere images. * p < 0.05; ** p < 0.01, n.s.: not significant, O/E: overexpression.