Figure 2.

Proximal tubule-specific loss of Sirt6 exacerbates UUO-induced tubular injury and fibrosis. Representative sections of kidneys from sham- and UUO-operated Sirt6^flox/flox; Ggt1-Cre^- (WT) and Sirt6^flox/flox; Ggt1-Cre⁺ (PT-Sirt6KO) mice were stained with Hematoxylin and Eosin (H&E) and Masson’s trichrome (MTC). Scale bar = 100 μm. The bar graph shows semi-quantitative scoring of tubular injury by H&E, area fractions (%) of tubulointerstitial fibrosis, and degree of interstitial collagen deposition stained by MTC in the sham and UUO-operated kidneys at a magnification of 200×. Ten randomly chosen, non-overlapping fields were quantified (n = 15 per group). Data are expressed as mean ± SD. *, p < 0.05 and ***, p < 0.001 versus WT sham or PT-Sirt6KO sham; ###, p < 0.001 versus WT UUO. Sham, sham-operated mice; UUO, unilateral ureteral obstruction; WT, wild-type; PT-Sirt6KO, proximal tubule-specific Sirt6 knockout.