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. 2022 May 9;14(9):2338. doi: 10.3390/cancers14092338

Figure 3.

Figure 3

MBOP promotes CRC progression through cell migration and proliferation. (A) Representative images of expressions of LINC01234ORF and MBOP in HCT116 and HCT15 after transfection of series plasmids pcDNA3.1+, pORF-FLAG, and pORFmut-FLAG. Data are presented as mean ± SD, ** p < 0.005, *** p < 0.0005. (B) Migration ability of cells transfected with series plasmids pcDNA3.1+, pORF-FLAG, and pORFmut-FLAG were detected with wound healing assay. (C) Migration ability of cells transfected with series plasmids pcDNA3.1+, pORF-FLAG, and pORFmut-FLAG were detected with transwell assay. (D) Colony formation assays of lentiviral transfected cell lines co-GFP, ORF-GFP, and ORFm-GFP. (E) The mRNA and protein expressions of MBOP from primary tumors resulted from in vivo plant of lentiviral transfected HCT116 cell lines co-GFP, ORF-GFP, and ORFm-GFP. Data are presented as mean ± SD, ** p < 0.005. (F) Mice bearing tumor fragments derived from primary tumors of (E), and the relative tumor volumes were recorded (n = 4). Data are presented as mean ± SD, **** p < 0.0001. The representative images of the immunohistochemistry assay are shown in the right panel.