Table 1.
The content of steroids in the T. × media KT hairy root line after elicitation with 100 µM MeJA.
| Compound | Content (µg/g D.W. ± SE) | |||||
|---|---|---|---|---|---|---|
| Free Forms | Esters | Glycosides | ||||
| Control | MeJA-Treated | Control | MeJA-Treated | Control | MeJA-Treated | |
| campesterol | 185.73 ± 5.77 a | 152.39 ± 9.32 b | 1.74 ± 0.08 a | 0.73 ± 0.06 b | 2.86 ± 0.11 a | 4.57 ± 0.05 b |
| cholesterol | 33.02 ± 4.12 a | 13.10 ± 1.92 b | 0.64 ± 0.05 a | 0.44 ± 0.03 b | 2.74 ± 0.10 a | 5.16 ± 0.05 b |
| isofucosterol | 47.52 ± 4.18 a | 9.14 ± 1.58 b | 2.07 ± 0.09 a | 1.12 ± 0.08 b | 2.01 ± 0.07 a | 3.18 ± 0.11 b |
| sitosterol | 1319.23 ± 61.29 a | 546.00 ± 18.20 b | 9.94 ± 0.72 a | 5.16 ± 0.19 b | 4.27 ± 0.12 a | 8.33 ± 0.36 b |
| stigmasterol | 61.50 ± 5.08 a | 627.85 ± 40.45 b | 2.35 ± 0.05 a | 2.30 ± 0.08 a | 1.55 ± 0.04 a | 12.34 ± 0.46 b |
| campestenone | 31.15 ± 3.88 a | 22.57 ± 0.42 b | ||||
| sitostenone | 120.01 ± 10.21 a | 132.45 ± 2.10 a | ||||
| stigmastanedione | 67.58 ± 5.96 a | 34.49 ± 1.19 b | ||||
| tremulone | 61.71 ± 4.66 a | 39.94 ± 6.25 b | ||||
| Sum of sterols | 1646.99 | 1348.47 | ||||
| Sum of ketones | 280.45 | 229.46 | ||||
| Total | 1927.44 | 1577.93 | 16.74 | 9.75 | 13.43 | 33.58 |
Results refer to hairy root dry weight and expressed as the mean ± SE of three independent samples. Results are compared statistically within categories (free forms, esters and glycosides); results not sharing a common letter are significantly different (p < 0.05).