Table 3.
The content of steroids in the T. × media ATMA (harboring the TXS transgene) hairy root line after elicitation with 100 µM MeJA.
| Compound | Content (µg/g D.W. ± SE) | |||||
|---|---|---|---|---|---|---|
| Free Forms | Esters | Glycosides | ||||
| Control | MeJA-Treated | Control | MeJA-Treated | Control | MeJA-Treated | |
| campesterol | 108.42 ± 5.51 a | 194.34 ± 5.63 b | 0.95 ± 0.05 a | 2.78 ± 0.07 b | 3.56 ± 0.08 a | 3.62 ± 0.03 a |
| cholesterol | 10.12 ± 0.54 a | 27.02 ± 1.65 b | 0.99 ± 0.04 a | 0.47 ± 0.07 b | 4.60 ± 0.10 a | 4.67 ± 0.05 a |
| isofucosterol | 12.19 ± 0.69 a | 47.02 ± 3.78 b | 0.75 ± 0.05 a | 1.84 ± 0.05 b | 1.63 ± 0.07 a | 1.41 ± 0.04 b |
| sitosterol | 695.44 ± 36.87 a | 650.58 ± 18.21 a | 4.20 ± 0.13 a | 11.58 ± 0.48 b | 10.35 ± 0.72 a | 7.98 ± 0.15 b |
| stigmasterol | 24.55 ± 2.73 a | 364.26 ± 26.39 b | 2.43 ± 0.08 a | 4.05 ± 0.13 b | 1.64 ± 0.06 a | 11.71 ± 0.19 b |
| campestenone | 23.08 ± 2.05 a | 50.09 ± 6.58 b | ||||
| sitostenone | 140.42 ± 11.08 a | 268.06 ± 10.18 b | ||||
| stigmastanedione | 37.86 ± 4.66 a | 71.12 ± 6.19 b | ||||
| tremulone | 34.79 ± 4.48 a | 67.05 ± 5.22 b | ||||
| Sum of sterols | 850.72 | 1283.22 | ||||
| Sum of ketones | 236.16 | 456.33 | ||||
| Total | 1086.88 | 1739.55 | 9.32 | 20.72 | 21.78 | 29.39 |
Results refer to hairy root dry weight and expressed as the mean ± SE of three independent samples. Results are compared statistically within categories (free forms, esters and glycosides); results not sharing a common letter are significantly different (p < 0.05).