Table 2.
Base Liquid ingredient formulation standard.
| Title | Ingredients (Article No., Manufacturer) |
Mass/Volume | Concentration |
|---|---|---|---|
| StockA | NaCl (S5886, sigma) KCl (p5405, sigma) KH2PO4 (p5655, sigma) MgCl2·6H2O (m2393, sigma) Na-lactate (l7900, sigma) Glucose (g7021, sigma) |
1.166 g 0.1068 g 0.0324 g 0.02 g 0.1232 mL 0.054 g |
997.6 mM 71.63 mM 11.90 mM 4.919 mM 0.616 (v/v)% 14.99 mM |
| Ultrapure water | 20 mL | ||
| StockB | NaHCO3 (S5761, sigma) | 0.42 g | 250.0 mM |
| Ultrapure water | 20 mL | ||
| StockC | Na Pyruvate (p4562, sigma) | 0.018 g | 32.72 mM |
| Ultrapure water | 5 mL | ||
| StockD | CaCl2·2H2O (c7902, sigma) | 0.1008 g | 171.4 mM |
| Ultrapure water | 5 mL | ||
| StockX | L-Glutamine (g3126, sigma) | 0.073 g | 99.90 mM |
| Ultrapure water | 5 mL | ||
| StockY | Sodium citrate (s1804, sigma) | 0.05 g | 34.00 mM |
| Ultrapure water | 5 mL | ||
| StockZ | Myo-Inositol (I7508, sigma) | 0.2495 g | 277.0 mM |
| Ultrapure water | 5 mL | ||