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. 2022 Apr 27;23(9):4842. doi: 10.3390/ijms23094842

Figure 1.

Figure 1

NGF modulates the expression of the main proteins involved in cholesterol homeostasis in U373 cells and enhances ApoE secretion in U373 culture medium. (A–G) Representative Western blot and densitometric analysis of SREBP-1, SREBP-2, HMGCR, LDLr, NPC1, ABCA1, and ApoE in U373 cells treated with vehicle (Ctrl) and NGF (100 ng/mL) for 48 h. n = 6 different experiments. Actin and vinculin were used as loading controls. (H) Quantification of ApoE levels (µg/mL) by ELISA assay in vehicle- and NGF-treated U373 culture medium. n = 3 different experiments. Data represent means ± SD. Statistical analysis was assessed by using unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001.