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. 2022 Apr 27;23(9):4842. doi: 10.3390/ijms23094842

Figure 5.

Figure 5

Effects of U373 conditioned medium on oxidative stress in neurons. Representative images in bright field and quantitative assessment of neuronal morphology of N1E-115. Cells were previously treated (+) or not (−) with rotenone (0.1 µM) for 16 h, and then cultured in fresh DMEM (Ctrl), in conditioned medium derived from control U373 (U373-Ctrl), NGF-pre-treated U373 (U373-NGF), and NGF-pre-treated U373 silenced for apoE (U373-NGF ApoE siRNA) for 48 h. n = 5 different experiments. Data represent means ± SD. Statistical analysis was assessed by using one-way ANOVA, followed by Tukey’s post hoc test. ** p < 0.01, *** p < 0.001.