Figure 2.

High-content screening of the cardiovascular compound library using the angiogenesis assay and hit identification. (a) Scatter-plot distribution showing the results of high-content screening. The lumen area and the total tube length were normalized to controls treated with vehicle (DMSO). Enhancer hits of the lumen area are indicated below and above the dotted lines (hit threshold was calculated as follows: median_(vehicle) + 4 × standard deviation_(vehicle)). Negative controls (are shown in blue positive controls in red and drug library molecules in green. (b) Calcium blockers (in red) were identified as enhancers of lumen formation from the library. (c) Representative fluorescent images from negative control (vehicle) and Lacidipine (enhancer hit for lumen area). Scale bar = 100 µm. (d) Dose–response curve demonstrating the effect of Lacidipine on lumen size using the angiogenesis assay. Data are expressed as mean ± SEM. n = 3 per concentration. The EC₅₀ was calculated as 250 nM.