Figure 3.

Phospho-p65 levels were induced by GSK101 in primary Müller cells. MIO-M1 cells were treated with increasing does of GSK101 for 24 h. Cell lysates were fractionated into nucleus and cytosol fractions and then subjected to Western blot. From 6 nM GSK101, p65 phosphorylation was significantly induced in both the cytosolic and nuclear fractions in a dose-dependent manner. GAPDH and Lamin B1 were served as the internal controls for cytosol and nucleus fractions, respectively. Asterisks indicate statistical significance. * p < 0.05, ** p < 0.01. n = 4.