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. 2022 May 6;23(9):5190. doi: 10.3390/ijms23095190

Figure 4.

Figure 4

Figure 4

GKS101-mediated phosphorylation of p65 attenuated by statins. MIO-M1 cells were treated either with GSK101 alone for 24 h or pretreated with (A) simvastatin, (B) atorvastatin, or (C) lovastatin for 6 h, followed by GSK101 treatment for 24 h. The cytosol and nucleus fractions were subjected to Western blot analysis. P65 phosphorylation significantly decreased in the nucleus after pretreatment with 0.2 nM of all three statins and decreased in the cytosol after pretreatment with 0.2 nM simvastatin, 1 nM atorvastatin, and 10 nM lovastatin. GAPDH and Lamin B1 were served as the internal controls for cytosol and nucleus fractions, respectively. Asterisks indicate statistical significance. * p < 0.05, ** p < 0.01. n = 4 for each figure.