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. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: Liver Int. 2022 Feb 15;42(4):829–841. doi: 10.1111/liv.15182

Figure 5: — In vitro 3D human NASH model was established by co-culture of human hepatocyte/ THP-1-derived macrophages/human stellate cells to form spheroid. (A) Representative phase contrast images of 3D spheroid; (B) Spheroid was treated with NASH inducing media for 5 days to induce inflammation and fibrosis. Gene expression was determined by qPCR; (C) Spheroid was treated with NASH inducing media for 5 days and then treated for additional 5 days in the presence of control IgG or anti-CD47 antibody (20 μg/ml). Gene expression was determined by qPCR. Data are represented as mean ± SE (n=3 separate experiments). *P<0.05; **P<0.01; ***P<0.001; (D) Representative immunofluorescent images from human NASH organoid (Blue = DAPI; Green = Collagen I; Red = αSMA). Scale bar = 200 μm.

Figure 5: — In vitro 3D human NASH model was established by co-culture of human hepatocyte/ THP-1-derived macrophages/human stellate cells to form spheroid. (A) Representative phase contrast images of 3D spheroid; (B) Spheroid was treated with NASH inducing media for 5 days to induce inflammation and fibrosis. Gene expression was determined by qPCR; (C) Spheroid was treated with NASH inducing media for 5 days and then treated for additional 5 days in the presence of control IgG or anti-CD47 antibody (20 μg/ml). Gene expression was determined by qPCR. Data are represented as mean ± SE (n=3 separate experiments). *P<0.05; **P<0.01; ***P<0.001; (D) Representative immunofluorescent images from human NASH organoid (Blue = DAPI; Green = Collagen I; Red = αSMA). Scale bar = 200 μm.