Table 2.
Primer Sequences for QPCR
| Gene | Primer sequence | Genes | Primer sequence |
|---|---|---|---|
| Mouse primers | |||
| FASN | 5’-TCCTGGAACGAGAACACGATCT-3’ 5’-GAGACGTGTCACTCCTGGACTTG-3’ |
SCD-1 | 5’-TTCTTGCGATACACTCTGGTGC-3’ 5’-CGGGATTGAATGTTCTTGTCGT-3’ |
| ACC | 5’-CCCAGCAGAATAAAGCTACTTTGG-3’ 5’-TCCTTTTGTGCAACTAGGAACGT-3’ |
SREBP1c | 5’-GGAGCCATGGATTGCACATT-3’ 5’-ACAAGGGTGCAGGTGTCACC-3’ |
| HMGCS | 5’-GACAAGAAGCCTGCTGCCATA-3’ 5’-CGGCTTCACAAACCACAGTCT-3’ |
HMGCR | 5’-TGCACGGATCGTGAAGACA-3’ 5’-GTCTCTCCATCAGTTTCTGAACCA-3’ |
| F4/80 | 5’-CTTTGGCTATGGGCTTCCAGTC-3’ 5’-GCAAGGAGGACAGAGTTTATCGTG-3’ |
CD11b | 5’-CGGAAAGTAGTGAGAGAACTGTTTC-3’ 5’-TTATAATCCAAGGGATCACCGAATTT-3’ |
| CD11c | 5’-CTGGATAGCCTTTCTTCTGCTG-3’ 5’-GCACACTGTGTCCGAACTC-3’ |
MCP-1 | 5’-CAGCCAGATGCAGTTAACGC-3’ 5’-GCCTACTCATTGGGATCATCTTG-3’ |
| IL-1β | 5’-TGGAGAGTGTGGATCCCAAGCAAT-3’ 5’-TGTCCTGACCACTGTTGTTTCCCA-3’ |
TNFα | 5’-AGCCGATGGGTTGTACCT-3’ 5’-TGAGTTGGTCCCCCTTCT-3’ |
| TGFβ | 5’-ACAATTCCTGGCGTTACC-3’ 5’-GGCTGATCCCGTTGATTT-3’ |
α-SMA | 5’-ATTGTGCTGGACTCTGGAGATGGT-3’ 5’-TGAGTCACGGACAATCTCACGCT-3’ |
| TIMP1 | 5’-TCTTGGTTCCCTGGCGTACTCT-3’ 5’-GTGAGTGTCACTCTCCAGTTTGC-3’ |
Col1a1 | 5’-TTCTCCTGGCAAAGACGGACTCAA-3’ 5’-AGGAAGCTGAAGTCATAACCGCCA-3’ |
| Col3a1 | 5’-TCCTAACCAAGGCTGCAAGATGGA-3’ 5’-ATCTAGATCCCGCCCTTGGTTTGT-3’ |
Col5a1 | 5’-TCTCTGTGTGTGTGCCAAGATGGA-3’ 5’-AGCCAGAGTCCATCCCACATTTCT-3’ |
| GAPDH | 5’-AACTTTGGCATTGTGGAAGG-3’ 5’-GGATGCAGGGATGATGTTCT-3’ |
||
| Human primers | |||
| GFAP α-SMA |
5’-GCAGAGATGATGGAGCTCAATGACC-3’ 5’-GTTTCATCCTGGAGCTTCTGCCTCA-3’ 5’-CCAGAGCCATTGTCACACAC-3’ 5’-CAGCCAAGCACTGTCAGG-3’ |
Col1a1 | 5’-GGATTCCAGTTCGAGTATGG-3’ 5’-CAGTGGTAGGTGATGTTCTG-3’ |
| TNFα | 5’-CACCACTTCGAAACCTGGGA-3’ 5’-AGGAAGGCCTAAGGTCCACT-3’ |
β-Actin | 5’-CATGTACGTTGCTATCCAGGC -3’ 5’-CTCCTTAATGTCACGCACGAT -3’ |
| IL-1β | 5’-CAACAGGCTGCTCTGGGATT-3’ 5’-CATGGCCACAACAACTGACG-3’ |
CD68 | 5’-GCTACATGGCGGTGGAGTACAA-3’ 5’-ATGATGAGAGGCAGCAAGATGG-3’ |