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. 2022 Apr 23;23(9):4688. doi: 10.3390/ijms23094688

Figure 5.

Figure 5

LPS-mediated gene expression. Cardiac gene expression analyzed by qPCR of wild type (WT) and PP2A overexpressing (PP2A-TG) mouse hearts after intraperitoneal application of LPS or NaCl solution as control (Ctr) after 7 h. Ordinates display relative mRNA expression in percentage of control, that is, WT hearts from mice treated with isotonic sodium chloride solutions alone (solvent control). (A) displays the mRNA for interleukin 1 beta (IL-1β), for interleukin 6 (IL-6) and for tumor necrosis factor alpha (TNFα). (B) displays the mRNA for atrial natriuretic peptide (ANP), for toll like receptor 4 (TLR4) and for cluster of differentiation 14 protein (CD14). Three days after LPS injection in (C) mRNA for interleukin 6 (IL-6) and tumor necrosis factor alpha (TNFα) as depicted in the ordinate. Note that after three days, expression of cytokine mRNAs nearly reached basal values. Data were normalized to GAPDH (A,B) or 18S RNA (C) mRNA expression and WT by the 2−ΔΔCT method. ★ p < 0.05 vs. Ctr, # p < 0.05 vs. WT.