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. 2022 Apr 25;14(9):2142. doi: 10.3390/cancers14092142

Figure 4.

Figure 4

Resveratrol maintains constant the dimensions of 3D ovarian cancer spheroids even in the presence of a pro-inflammatory stimulus. OVCAR3 cells were grown as 3D spheroids. On day 0, cells were exposed to 100 μM RV and re-treated (on days 1 and 3 with 20 μM RV; on days 5 and 7, 10 μM RV was added alone or in combination with 50 ng/mL IL-6 or replaced by IL-6) up to 10 days. 3D spheroid dimensions were determined with ImageJ software. Data represent the average ± S.D. calculated for three different fields per condition in three separate experiments. (A) Time-course phase-contrast photos of OVCAR3 3D spheroids. (B) Growth curves represent the dimensions of 3D spheroids during the experimental timeline. (C) Graph reporting the quantification of 3D spheroid areas on day 10. Statistical analysis was performed by using GraphPad Prism 5.0 software. Bonferroni’s multiple comparison test after one-way ANOVA was employed. Significance was considered as follows: * p < 0.05.