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. 2022 Apr 25;14(9):2142. doi: 10.3390/cancers14092142

Figure 9.

Figure 9

miR-1305 inhibits while anti-miR-1305 induces ARH-I-mediated autophagy. (A) The co-immunoprecipitation of ARH-I binding partners of the pro-autophagic interactome. Cells were cultured in 2D and transfected with miR-1305 mimic or anti-miR-1305 for 72 h. The quantification of the protein–protein interactions is included. Data are representative of three independent replicates. (B) Western blotting of p62 and LC3 for determining the autophagy flux in OVCAR3 cells transfected with miR-1305 or anti-miR-1305 for 72 h. Densitometric analysis of Western blotting representative of three independent experiments is included. (C) OVCAR3 cells were transfected with anti-miR-1305, and after 16 h the cells were treated with 50 ng/mL IL-6 and further cultured for 24 h. Western blotting of p62, ARH-I and LC3. The densitometric analysis of Western blotting representative of three independent experiments is included. (D) OVCAR3-GFP-LC3 cells were plated on coverslips, treated with 50 ng/mL IL-6, or transfected with miR-1305 or anti-miR-1305 for 72 h. Coverslips were imaged with a fluorescence microscope. Scale bar = 20 µm; magnification = 63×.