Table 1.

Cost items included for the estimation of costs of the West Nile Virus (WNV) integrated surveillance system in Lombardy, 2014–2018.

Item	Description	Details
Human surveillance	-Cost of diagnosis of West Nile neurologic disease in suspect cases and hospitalization	Personnel cost not included for laboratory analysis and included for hospitalizations
Entomological surveillance	-Mosquito collection -Screening: PCR on each mosquito pool -Confirmation of each positive pool: PCR, cell culture, and sequencing	Personnel cost included
Ornithological surveillance	-Wild bird collection -Screening: PCR on pool of organs (brain spleen heart and kidney) of individual wild birds	Personnel cost included
Equinesurveillance	-Cost of diagnosis of WNV neurologic disease in suspect horses -Screening: IgM and IgG ELISA, competitive ELISA on serum, PCR on EDTA-blood of alive and dead suspect horses	Personnel costnot included
Blood testing	-Nucleic acid amplification test (NAT) on each blood donor sample	Personnel cost included