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. 2022 Apr 29;23(9):4948. doi: 10.3390/ijms23094948

Figure 1.

Figure 1

Cd increased the degree of apoptosis in granulosa cells. (A) Analysis of the viability of cells exposed to Cd using CCK-8. (B) Flow cytometry analysis of the degree of apoptosis in the control and Cd groups. The TUNEL assay detected apoptotic cells in the control and Cd groups. Green fluorescence represents apoptotic cells. (C) RT-qPCR results revealed the transcript levels of these genes after Cd treatment. (D) Protein levels after Cd treatment were analyzed by Western blotting. * p < 0.05; ** p < 0.01.