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. 2022 Apr 29;23(9):4948. doi: 10.3390/ijms23094948

Figure 2.

Figure 2

Figure 2

Cd-induced oxidative stress in granulosa cells. (AC) RT-qPCR results revealed the transcript levels of CAT, SOD, and NQO1 after Cd treatment. (D) RT-qPCR results revealed the transcript levels of Nrf2 after Cd treatment. (EH) ELISA results revealed the intracellular levels of CAT, SOD, NQO1, and MDA after Cd treatment. (I) Intracellular reactive oxygen species (ROS) was measured using flow cytometry analysis (DHE) in the control and Cd groups. (J) Nrf2 protein levels were analyzed by Western blotting after Cd treatment. * p < 0.05; ** p < 0.01.