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. 2022 Apr 26;23(9):4782. doi: 10.3390/ijms23094782

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Generation and validation of transgenic SH-SY5Y clones. pcDNA™4/TO (A) and pENTR™/H1/TO (B) vectors used for cathepsin D overexpression and silencing, respectively. Below, western blot analysis of CD expression is shown. (C) Western blot of cathepsin D in SH-SH5Y Sham, 486 (KD-CD) and Over CD clones. Densitometry of the bands is reported in the histogram.