Figure 1.

Soluble α-Klotho serum levels across age groups (i.e., 18–34 years (n = 167) vs. 35–54.9 years (n = 88) vs. 55–85 years (n = 91)) for all participants (A) and segmented by sex (B). (A): One-way analysis of variance to compare soluble α-Klotho serum levels across age groups (* p < 0.05, ** p < 0.01, and *** p < 0.001). (B): Student’s unpaired t-test including sex as a fixed variable (men vs. women) to determine differences in soluble α-Klotho serum levels in each age group. Values are expressed as means ± reference interval (5th–95th percentile).