Figure 3.

Loss of scleraxis attenuates glutaminolysis gene expression. (A,B) Cardiac fibroblasts were isolated from wild-type (WT) or scleraxis knockout (KO) mice and assayed for the expression of GLS1 (A) and GDH1 (B) by qPCR. (C,D) P1 rat cardiac fibroblasts were transfected with adenovirus encoding small hairpin RNA targeting LacZ (AdshLacZ) or scleraxis (AdshScx) for 72 h, with or without 10 ng/mL TGFβ₁ addition after 48 h, and assayed by qPCR for expression of scleraxis (C) and GLS1 (D). Statistical analysis was by the two-tailed Student’s t-test (A,B) or by one-way ANOVA with the Tukey post hoc test (C,D) (n = 3). ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus WT, or AdshLacZ; # p < 0.05 versus AdshScx. NS, not significant.