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. 2022 Apr 28;23(9):4922. doi: 10.3390/ijms23094922

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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EdU-coupled fluorescence-intensity analysis of distinct cell-cycle characteristics. (a) The proportion of EdU-positive cells in CD-sorted populations of NSCLC cells 24–48 h after irradiation. (b) Using the ImageXpress Micro XL High-Content Screening System, we analyzed EdU fluorescence in PGCC 24–48 h after 0 Gy (blue dots) or 5 Gy (red dots) exposure The nuclear area of 400 nm was used as a threshold for PGCC cells according to the literature. Green circles demonstrate EdU^low area of “quiescent” cells. Data are means ± SD of three independent experiments. Statistical significance is denoted by asterisks, where: **** p < 0.0001.

EdU-coupled fluorescence-intensity analysis of distinct cell-cycle characteristics. (a) The proportion of EdU-positive cells in CD-sorted populations of NSCLC cells 24–48 h after irradiation. (b) Using the ImageXpress Micro XL High-Content Screening System, we analyzed EdU fluorescence in PGCC 24–48 h after 0 Gy (blue dots) or 5 Gy (red dots) exposure The nuclear area of 400 nm was used as a threshold for PGCC cells according to the literature. Green circles demonstrate EdU^low area of “quiescent” cells. Data are means ± SD of three independent experiments. Statistical significance is denoted by asterisks, where: **** p < 0.0001.