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. 2022 Apr 22;23(9):4630. doi: 10.3390/ijms23094630

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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m6A-containing mRNAs related to gene expression and important biological pathways in female and male An. sinensis. (a) The mRNA expression levels in females (left) and males (right) containing m6A peaks. Genes were divided into three categories according to the numbers of m6A sites in each gene. non-m6A gene (number of m6A site = 0), low m6A gene (number of m6A site < 3), high m6A gene (number of m6A site ≥ 3). Genes were also divided into five categories (3′ UTR, 5′ UTR, both 3′ and 5′ UTR, 1st exon and other exon) according to the annotation of the m6A peak in each gene. (b) Differentially expressed genes in female and male An. sinensis. Genes with high m6A modification and genes with m6A peaks at both 3′ and 5′ UTR are highlighted with colorful dots. (c) GO enrichment of sex-specific m6A genes in female and male An. sinensis. Female-m6A, m6A genes in female; Male-m6A, m6A genes in male; female high m6A genes, genes with m6A sites ≥ 3 in females; Male high m6A genes, genes with m6A sites ≥ 3 in males.

m6A-containing mRNAs related to gene expression and important biological pathways in female and male An. sinensis. (a) The mRNA expression levels in females (left) and males (right) containing m6A peaks. Genes were divided into three categories according to the numbers of m6A sites in each gene. non-m6A gene (number of m6A site = 0), low m6A gene (number of m6A site < 3), high m6A gene (number of m6A site ≥ 3). Genes were also divided into five categories (3′ UTR, 5′ UTR, both 3′ and 5′ UTR, 1st exon and other exon) according to the annotation of the m6A peak in each gene. (b) Differentially expressed genes in female and male An. sinensis. Genes with high m6A modification and genes with m6A peaks at both 3′ and 5′ UTR are highlighted with colorful dots. (c) GO enrichment of sex-specific m6A genes in female and male An. sinensis. Female-m6A, m6A genes in female; Male-m6A, m6A genes in male; female high m6A genes, genes with m6A sites ≥ 3 in females; Male high m6A genes, genes with m6A sites ≥ 3 in males.