Figure 3.

SRSF5 restored the inhibition mediated by AO2+1. (A). Characterization of splicing regulatory factors. The nucleotide sequence around the AO2+1 target site was analyzed for splicing enhancer and silencer sequences with SpliceAid2. The output graph is shown. The nucleotide sequences of exon 1 are described in uppercase letters. The binding location of SFRF5 is indicated by a green bar, and the binding strength is represented by the height. A red bar indicates the target site of AO2+1. (B). CRL–2061 cells were transfected with different doses of SRSF5 plasmid with or without AO2+1. A fragment extending from exon 1 to exon 3 was RT–PCR amplified. Electrophoretograms of the amplified products are shown (bottom). The amount of product was calculated and is expressed as the ratio of MSTN to GAPDH. The ratio increased with increasing plasmid dose and reached 0.8 at 1 μg of the plasmid. * = p < 0.05, *** = p < 0.001 vs. 0 μg SRSF5 and no ASO treatment.