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. 2022 Apr 30;23(9):5016. doi: 10.3390/ijms23095016

Figure 5.

Figure 5

Dose-dependent action of KMM001 on splicing of the MSTN gene. (A) KMM001 was added to the culture medium of CRL-2061 cells at the indicated concentrations, and the cells were cultured for 1 day. Fragments of exons 1 to 3 and intron 2 to exon 3 of MSTN pre-mRNA were RT–PCR amplified. Electrophoretograms of the PCR products are shown. (B). The MSTN/GAPDH ratio was calculated for each designated concentration after semiquantification of the amplified products using an Agilent Bioanalyzer. The ratio plotted against the concentration of KMM001 is shown (upper). The ratio decreased dose dependently from 0 to 100 nM KMM001 and reached its lowest value at 100 nM. Next, the pre-MSTN/GAPDH ratio was calculated for each designated concentration. The ratio plotted against the concentration of KMM001 is shown (lower). The ratio increased dose dependently from 0 to 100 nM KMM001 and reached a plateau at 100 nM. ** = p < 0.01, *** = p < 0.001 vs. 0 nM KMM001.