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. 2022 Apr 30;23(9):5016. doi: 10.3390/ijms23095016

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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KMM001 enhanced the proliferation of human myoblasts. (A). KMM001 was added to the culture medium of immortalized human myoblasts. In a CCK-8 assay, the absorbance increased in treated (red line) and nontreated (gray line) cells. Notably, the absorbance of the cells treated with KMM001 was significantly higher at third day than that of the nontreated cells (146%, p < 0.001). (B). Direct cell counting using a fluorescence microscope showed that the numbers of cells increased over time in both the treated (red line) and nontreated (gray line) groups. Notably, the number of KMM001-treated cells was significantly higher than that of nontreated cells at third day (138%, p < 0.01). * = p < 0.05, ** = p < 0.01, *** = p < 0.001 vs. 0 day.