Figure 3.

Nuci increased energy metabolism in HFD-fed mice. Seven-weeks male C57BL/6J mice were fed with ND, HFD, HFD supplemented with Nuci and HFD with subcutaneous injection of Lira at 200 μg/kg/day for 12 weeks. General pictures of mice in ND, HFD, HFD-Nuci, and HFD-Lira group were presented (a). After 11 weeks of treatment, mice in the ND, HFD, and HFD-Nuci group were placed in metabolic cages at 22 °C at 12 h light–dark cycle. VO₂ (b), VCO₂ (d) were detected and quantification of VO₂ (c), VCO₂ (e), RER (f), EE (g), and locomotor activity (h) during both the light and dark cycle were determined by Promethion. Data were presented as mean ± SE, n = 4 in each group. * p < 0.05 vs. the HFD group.