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. 2022 Apr 30;14(9):1898. doi: 10.3390/nu14091898

Figure 4.

Figure 4

Nuci ameliorated hepatic steatosis by activating AMPK phosphorylation in liver of HFD-fed mice. Seven-weeks male C57BL/6J mice were fed with ND, HFD, HFD supplemented with Nuci and HFD with subcutaneous injection of Lira at 200 μg/kg/day for 12 weeks. H&E-staining (a) and Oil-red O staining (b) images of representative sections of mice liver in the ND, HFD, HFD-Nuci, and HFD-Lira group; photographs were taken at 200× magnification. NAFLD scores of the four groups according to a general NAFLD scoring system for rodent models (c). The mRNA expressions of lipogenesis-related genes SREBP1, FAS, and ACC (d), lipolysis-related genes HSL and ATGL (e), fatty acid oxidation-related genes PPARα and CPT1 (f), and adipokines FGF21 and ZAG (g) were determined by RT-qPCR. The protein levels of p-AMPK, t-AMPK and β-actin were analyzed by Simple western (h). the expression of p-AMPK was normalized against t-AMPK (i). Data were presented as mean ± SE, n = 12 in each group. * p < 0.05 vs. the HFD group.