Table 3.
The average number of fragments obtained from eleven selective primer combinations and sequences to detect AFLPs among O. oeni strains isolated from Grenache wines. Bold type indicates the additional bases used for the selective primer.
| Primer Pair | Sequences of Selective Primers | Fragment No. |
|---|---|---|
| MC-HT FAM | MC 5′-GATGAGTCCTGAGTAAC-3′ HT 5′-GACTGCGTACCAGCTTT-3′ |
57–81 |
| MA-HT FAM | MA 5′-GATGAGTCCTGAGTAAA-3′ HT 5′-GACTGCGTACCAGCTTT-3′ |
76–85 |
| MT-HT FAM | MT 5′-GATGAGTCCTGAGTAAT-3′ HT 5′-GACTGCGTACCAGCTTT-3′ |
70–85 |
| MG-HT FAM | MG 5′-GATGAGTCCTGAGTAAG-3′ HT 5′-GACTGCGTACCAGCTTT-3′ |
57–64 |
| MA-HC FAM | MA 5′-GATGAGTCCTGAGTAAA-3′ HC 5′-GACTGCGTACCAGCTTC-3′ |
54–62 |
| MT-HC FAM | MT 5′-GATGAGTCCTGAGTAAT-3′ HC 5′-GACTGCGTACCAGCTTC-3′ |
52/74 |
| MC-HC FAM | MC 5′-GATGAGTCCTGAGTAAC-3′ HC 5′-GACTGCGTACCAGCTTC-3′′ |
57–75 |
| MG-HC FAM | MG 5′-GATGAGTCCTGAGTAAG-3′ HC 5′-GACTGCGTACCAGCTTC-3′ |
63–66 |
| HG-MA HEX | HG 5′-GACTGCGTACCAGCTTG-3′ MA 5′-GATGAGTCCTGAGTAAA-3 |
52–78 |
| HG-MT HEX | HG 5′-GACTGCGTACCAGCTTG-3′ MT 5′-GATGAGTCCTGAGTAAT-3 |
41–49 |
| HA-MT HEX | HA 5′-GACTGCGTACCAGCTTA-3′ MT 5′-GATGAGTCCTGAGTAAT-3 |
34–49 |