Figure 2.

Examples of the different phenotypes detected via the histochemical Lugol-Iodine staining of microtuber sections. WT: wild-type (Yukon Gold, strain TXYG79) tuber showing typical blue staining of starch indicative of normal amylose/amylopectin ratio; T1-27: wherein Target site 1 was targeted for mutation, showing small sectors of complete knockout phenotype surrounded by largely blue-colored tuber section; T1-32: wherein Target site 1 was targeted for mutation, but does not show the knockout phenotype; T2-7: wherein Target site 2 was targeted for mutation showing complete knockout phenotype, i.e., reddish-brown-colored staining throughout the tuber section due to the absence of amylose.