Figure 1.

Flowchart for this paper. The main framework includes immune repertoire construction and single-cell RNA-seq data analysis. During the process of immune repertoire construction, bulk RNA-seq data were used as inputs for TRUST4 algorithm. After building both T-cell receptor and B-cell receptor repertoires, we used the extracted information to perform further statistical analysis. During the process of single cell RNA-seq data analysis, we utilized the original data to perform cell clustering and compared the proportions of various of immune cell types under SLE, RA and HC conditions. Finally, DEGs identification and functional enrichment analysis were conducted for different immune cell types, respectively, under SLE, RA and HC conditions. Cell–cell interaction analysis of MIF and GALECTIN family signaling pathways was also implemented between several immune cell types.