Figure 4.

Trappc9 loss impacts the expression of rab proteins and autophagy in ASCs. Post-nuclear supernatants prepared from WT and trappc9-deficient (KO) ASCs at passage-4 were subjected to SDS-PAGE and Western blot analysis with antibodies for indicated rab proteins (A,B) and proteins related to autophagy (C,D). GAPDH was a loading control. Blot analyses shown in (A,C) were from one of three independent experiments. Densitometry quantification of signal intensities for each of the indicated proteins were graphed and shown in (C,D), respectively. Expression levels of indicated proteins were normalized with signal intensities of the corresponding GAPDH. Star symbols beside gel graphs indicated proteins cross-reactive to the corresponding antibodies. Data are Mean ± SD. Each symbol in (B,D) represents one experiment. Unpaired two-tailed Student’s t-test: * p <0.05; ** p < 0.01; n/s, no significance.