Fig. 3.
The protective effect of curcumin on OLV-induced ALI is related to Prdx6. A Western blot analysis of representative inflammatory factor levels, among which IL-6, IL-1β, and TNF-α are the classic proinflammatory factors and IL-10 is the anti-inflammatory factor in each group. B ELISA analysis of MDA levels in each group. C ELISA analysis of SOD levels in each group. D ROS levels were measured by DCFH-DA staining in each group. Scale bar = 100 μm. E Western blot analysis images of Prdx6 in each group. F qRT–PCR analysis of Prdx6 in each group. G Immunofluorescence analysis of Prdx6 in each group. DAPI is shown in blue and represents the nucleus. Scale bar = 100 μm. In the control group (control): cells were pretreated with the DMSO vehicle alone for 2 h, then changed to a serum-free medium for 48 h; in the curcumin group (cur): cells were pretreated with 40 μg/ml curcumin + DMSO for 2 h, then changed to serum-free medium for 48 h; in the serum group (serum): the cells were intervened with 20% OLVafter serum for 48 h; in the curcumin + serum group (cur + serum): the cells were first pretreated with 40 μg/ml curcumin and DMSO, then changed to 20% OLVafter serum for 48 h. The results are shown as the means ± SD of 3 individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001