Fig. 4.
Prdx6 mediated the protective function of curcumin in OLV-induced ALI by regulating the NF-κB pathway. A The levels of NF-κB signaling markers in each group were determined via Western blot. B Western blot analysis of Prdx6 expression in A549 cells transfected with either Prdx6-siRNA or NC-siRNA. C qRT–PCR analysis of Prdx6 expression in A549 cells transfected with either Prdx6-siRNA or NC-siRNA. D The expression of representative inflammatory factors and P65 in Prdx6-siRNA- or NC-siRNA-transfected cells with or without SC75741. E ROS levels were measured by DCFH-DA staining in cells transfected with either Prdx6-siRNA or NC-siRNA. Scale bar = 100 μm. F ELISA analysis of oxidative stress factor levels (MDA and SOD) in cells transfected with either Prdx6-siRNA or NC-siRNA. G The protein levels of NF-κB signaling markers in cells transfected with either Prdx6-siRNA or NC-siRNA. In the control group (control): the cells were pretreated with the DMSO vehicle alone for 2 h, then changed to a serum-free medium for 48 h; curcumin group (cur): the cells were pretreated with 40 μg/ml curcumin + DMSO for 2 h, then changed to serum-free medium for 48 h; serum group (serum): the cells were intervened with 20% OLVafter serum for 48 h; curcumin + serum group (cur + serum): the cells were first pretreated with 40 μg/ml curcumin and DMSO, then changed to 20% OLVafter serum for 48 h. The results are shown as the means ± SD of 3 individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001