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. 2022 Apr 27;11(9):1468. doi: 10.3390/cells11091468

Figure 5.

Figure 5

ANRIL/p15 promotes SIG in mice models. (A) The expression of p15 protein accumulated in mice anterior segment samples after exposure to DEX. p15 knockdown decreased p15 intraocular protein amount. The p15 level in the DEX + sip15 group was comparable to the control mice, suggested by IF. TM region is outlined with white dashes. n = 5 per group. (B) The IOP significantly increased in DEX-treated mice, starting from 15.3 mmHg at 4 weeks and continually increasing to 23.4 mmHg at 12 weeks. Intraocular ANRIL depletion presented a consistent increased IOP pattern (IOP = 22.1 ± 0.89 mmHg at 12 weeks, compared with control mice, p < 0.05). When p15 was knocked-down, IOP elevated in the very beginning period with DEX exposure (0–6 weeks) and then continually recovered to a normal level. Arrow indicates the starting of intracameral siRNA delivery on Day 21. Notes: CON = control; siANRIL = ANRIL knock-down; DEX = dexamethasone; sip15 = p15 knock-down. Scale bar = 10 μm.