Table 1.
Variables and levels used in the Box–Behnken design.
| Factors Level (Code) | Response | |||
|---|---|---|---|---|
| Run Order | Amplitude | Ethanol | Temperature | mg Trolox/gDM |
| 7 | 20 (−1) | 0 (−1) | 47.5 (0) | 4.52 |
| 6 | 100 (+1) | 0 (−1) | 47.5 (0) | 4.18 |
| 14 | 20 (−1) | 100 (+1) | 47.5 (0) | 6.54 |
| 9 | 100 (+1) | 100 (+1) | 47.5 (0) | 7.35 |
| 8 | 20 (−1) | 50 (0) | 25 (−1) | 17.53 |
| 2 | 100 (+1) | 50 (0) | 25 (−1) | 17.24 |
| 1 | 20 (−1) | 50 (0) | 70 (+1) | 24.78 |
| 4 | 100 (+1) | 50 (0) | 70 (+1) | 21.98 |
| 15 | 60 (0) | 0 (−1) | 25 (−1) | 5.62 |
| 3 | 60 (0) | 100 (+1) | 25 (−1) | 3.25 |
| 10 | 60 (0) | 0 (−1) | 70 (+1) | 5.30 |
| 11 | 60 (0) | 100 (+1) | 70 (+1) | 7.19 |
| 13 | 60 (0) | 50 (0) | 47.5 (0) | 20.79 |
| 12 | 60 (0) | 50 (0) | 47.5 (0) | 19.11 |
| 5 | 60 (0) | 50 (0) | 47.5 (0) | 21.18 |