TABLE 2.
Properties of purified TodC1 and ISPTOLa
| Protein | Absorption spectra, λmax (ɛ, mM−1, cm−1)b
|
Mean ± SD content (g-atoms mol−1) of:
|
EPR spectrae
|
|||||
|---|---|---|---|---|---|---|---|---|
| Oxidized | Reduced | Ironc | Acid-labile sulfided | Oxidized | Reduced
|
|||
| gx | gy | gz | ||||||
| TodC1 | 325 (10.33), 446 (6.67), 558 (3.67) | 376 (5.60), 526 (2.73) | 1.98 ± 0.14 | 1.91 ± 0.16 | Absent | 1.76 | 1.91 | 2.01 |
| ISPTOL | 325 (11.03), 446 (5.24), 558 (2.76) | 376 (4.93), 526 (2.50) | 2.02 ± 0.19 | 1.90 ± 0.08 | Absent | 1.76 | 1.91 | 2.01 |
a
ISPTOL was purified as described previously. The N-terminal sequence of purified TodC1 is MNQTDTSPIR.
b
For TodC1, based on α; for ISPTOL, based on αβ.
c
Determined by the method of Zabinski et al. (34).
d
Determined by the method of Beinert (1).
e
Recorded at 77 K with a Bruker ESP300 spectrometer with the following settings: 5.02 mW of microwave power, a 3,600-G centerfield, a 9.29-GHz microwave modulation frequency, a 42-s sweep time, and a receiver gain of 1.0 × 105.